anti cd3ε af647 (Bio-Rad)
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Anti Cd3ε Af647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd3/Rat+anti+Human+CD3%3AAlexa+Fluor+647/pmc13628097-131-10-11
Average 93 stars, based on 24 article reviews
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Isolation:Article Title: Loss of Endothelial YAP/TAZ Reduces the Size of Chronic Stroke Lesions and Alters the Endothelial Environment Article Snippet: .. Primary antibodies were applied in the following concentrations: anti- Iba1 (rabbit, Fujifilm Wako, 019- 19741) 1:500; anti- Ki67 (rat, eBioscience, # 14- 5698- 82) 1:500; anti- NeuN (mouse, Merck, #MAB377) 1:200; anti- ERG (rabbit, Abcam, #ab92513) 1:100; anti- GFP (goat, Origine, #R1091P) 1:100; isolectin B4 (GS- IB4)—Alexa647 (isolated from Griffonia simplicifolia, Thermo Fisher Scientific, #I32450) 1:100; anti- GLUT1 (rabbit, Calbiochem, #400060) 1:100; antiCD31 (goat, bio- techne, #AF3628) 1:100; anti- YAP1 (rabbit, Novus, #NB110- 58358) 1:100; anti- Ly6G (rat, BD Biosciences, #551459) 1:100; and Staining:Article Title: Pharmacological inhibition of tyrosine protein-kinase 2 reduces islet inflammation and delays type 1 diabetes onset in mice. Article Snippet: .. The slides were then stained with anti-insulin (AlexaFluor 488, 1:50; R&D Systems, catalogue #IC1417G, RRID:AB_3654708), anti-B220 (AlexaFluor 594, 1:150; BioLegend, catalogue #103254, RRID:AB_ 2563229)), and Article Title: Enhancing anti-EGFRvIII CAR T cell therapy against glioblastoma with a paracrine SIRPγ-derived CD47 blocker Article Snippet: For antigen retrieval, slides were exposed to pre-heated 1X Citrate Buffer (#C9999, Sigma-Aldrich, USA), followed by a washing step with PBS for 10 min. Tissue sections were permeabilized in PBS with 0.2% Tween-20 (#P5927, Sigma-Aldrich, USA) twice for 15 min. After that, the slides were washed with PBS twice for 10 min, followed by blocking for 1 h in a dark, humid chamber with 10% normal donkey serum (#017-000-121, Jackson ImmunoResearch, USA) diluted in PBS. .. Afterward, the slides were stained in different cycles overnight at 4 °C with the following antibodies: Article Title: Pharmacological inhibition of tyrosine protein-kinase 2 reduces islet inflammation and delays type 1 diabetes onset in mice Article Snippet: .. The slides were then stained with anti-insulin (AlexaFluor 488, 1:50; R&D Systems, catalogue #IC1417G, RRID: AB_3654708 ), anti-B220 (AlexaFluor 594, 1:150; BioLegend, catalogue #103254, RRID: AB_2563229 )), and Article Title: DPP4-inhibition reduces pro-inflammatory cytokine production by alpha-beta and gamma-delta T cells in vitro and in the biliary atresia mouse model Article Snippet: .. Liver sections were processed as previously described with antibodies rat anti-mouse GR-1, rat anti-mouse F4/80 and Article Title: DPP4-inhibition reduces pro-inflammatory cytokine production by alpha-beta and gamma-delta T cells in vitro and in the biliary atresia mouse model. Article Snippet: .. Liver sections were processed as previously described with antibodies rat anti-mouse GR-1, rat anti-mouse F4/80 and Incubation:Article Title: ETS1 Orchestrates a Hybrid EMT Program Driving in vivo Metastasis and Immune Evasion Article Snippet: Slides were blocked with Rodent Block M (BioCareMedical, RBM961) for 30 minutes at room temperature. .. Slides were incubated with an Fluorescence:Article Title: DPP4-inhibition reduces pro-inflammatory cytokine production by alpha-beta and gamma-delta T cells in vitro and in the biliary atresia mouse model Article Snippet: .. Liver sections were processed as previously described with antibodies rat anti-mouse GR-1, rat anti-mouse F4/80 and Article Title: DPP4-inhibition reduces pro-inflammatory cytokine production by alpha-beta and gamma-delta T cells in vitro and in the biliary atresia mouse model. Article Snippet: .. Liver sections were processed as previously described with antibodies rat anti-mouse GR-1, rat anti-mouse F4/80 and |
