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anti cd3ε af647  (Bio-Rad)


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    Structured Review

    Bio-Rad anti cd3ε af647
    Anti Cd3ε Af647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd3/Rat+anti+Human+CD3%3AAlexa+Fluor+647/pmc13628097-131-10-11
    Average 93 stars, based on 24 article reviews
    anti cd3ε af647 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Loss of Endothelial YAP/TAZ Reduces the Size of Chronic Stroke Lesions and Alters the Endothelial Environment
    Article Snippet: .. Primary antibodies were applied in the following concentrations: anti- Iba1 (rabbit, Fujifilm Wako, 019- 19741) 1:500; anti- Ki67 (rat, eBioscience, # 14- 5698- 82) 1:500; anti- NeuN (mouse, Merck, #MAB377) 1:200; anti- ERG (rabbit, Abcam, #ab92513) 1:100; anti- GFP (goat, Origine, #R1091P) 1:100; isolectin B4 (GS- IB4)—Alexa647 (isolated from Griffonia simplicifolia, Thermo Fisher Scientific, #I32450) 1:100; anti- GLUT1 (rabbit, Calbiochem, #400060) 1:100; antiCD31 (goat, bio- techne, #AF3628) 1:100; anti- YAP1 (rabbit, Novus, #NB110- 58358) 1:100; anti- Ly6G (rat, BD Biosciences, #551459) 1:100; and anti- CD3 (rat, Bio- RAD, #MCA1477T) 1:100. .. Immunohistochemistry followed the peroxidase method with anti- rabbit IgG biotinylated secondary antibody (1:250, dianova, #711- 065- 152), ABC Elite reagent (Biozol, #VEC- PK- 6100), and diaminobenzidine (Sigma, #D4293) as chromogen.

    Staining:

    Article Title: Pharmacological inhibition of tyrosine protein-kinase 2 reduces islet inflammation and delays type 1 diabetes onset in mice.
    Article Snippet: .. The slides were then stained with anti-insulin (AlexaFluor 488, 1:50; R&D Systems, catalogue #IC1417G, RRID:AB_3654708), anti-B220 (AlexaFluor 594, 1:150; BioLegend, catalogue #103254, RRID:AB_ 2563229)), and anti-CD3 (AlexaFluor 647, 1:200; BioRad, catalogue #MCA1477A647, RRID:AB_10841760) 7 8 antibodies. .. In addition, DSP antibody barcode collections including immune cell core profiling, immune cell activation, and immune cell typing (nanoString) were added to the slides and incubated overnight at 4 ◦C.

    Article Title: Enhancing anti-EGFRvIII CAR T cell therapy against glioblastoma with a paracrine SIRPγ-derived CD47 blocker
    Article Snippet: For antigen retrieval, slides were exposed to pre-heated 1X Citrate Buffer (#C9999, Sigma-Aldrich, USA), followed by a washing step with PBS for 10 min. Tissue sections were permeabilized in PBS with 0.2% Tween-20 (#P5927, Sigma-Aldrich, USA) twice for 15 min. After that, the slides were washed with PBS twice for 10 min, followed by blocking for 1 h in a dark, humid chamber with 10% normal donkey serum (#017-000-121, Jackson ImmunoResearch, USA) diluted in PBS. .. Afterward, the slides were stained in different cycles overnight at 4 °C with the following antibodies: anti-CD3 (clone CD3-12; #MCA1477, Bio-Rad, USA), anti-CD206 (clone E6T5J; #24595S, Cell Signaling Technology, USA), anti-EGFRvIII (clone RM419; #MA5-36216, ThermoFisher Scientific, USA), anti-GFAP (clone D1F4Q; #12389S, Cell Signaling Technology, USA), anti-IBA1 (polyclonal; #NB100-1028, Novus Biologicals, USA), anti-Ki67 (clone SolA15; #14-5698-82, ThermoFisher Scientific, USA) and anti-TMEM119 (clone 28-3; #ab209064, Abcam, UK). ..

    Article Title: Pharmacological inhibition of tyrosine protein-kinase 2 reduces islet inflammation and delays type 1 diabetes onset in mice
    Article Snippet: .. The slides were then stained with anti-insulin (AlexaFluor 488, 1:50; R&D Systems, catalogue #IC1417G, RRID: AB_3654708 ), anti-B220 (AlexaFluor 594, 1:150; BioLegend, catalogue #103254, RRID: AB_2563229 )), and anti-CD3 (AlexaFluor 647, 1:200; Bio-Rad, catalogue #MCA1477A647, RRID: AB_10841760 ) antibodies. .. The slides were then stained with anti-insulin (AlexaFluor 488, 1:50; R&D Systems, catalogue #IC1417G, RRID: AB_3654708 ), anti-B220 (AlexaFluor 594, 1:150; BioLegend, catalogue #103254, RRID: AB_2563229 )), and anti-CD3 (AlexaFluor 647, 1:200; Bio-Rad, catalogue #MCA1477A647, RRID: AB_10841760 ) antibodies.

    Article Title: DPP4-inhibition reduces pro-inflammatory cytokine production by alpha-beta and gamma-delta T cells in vitro and in the biliary atresia mouse model
    Article Snippet: .. Liver sections were processed as previously described with antibodies rat anti-mouse GR-1, rat anti-mouse F4/80 and anti-CD3 (Clone: 145-2C11) (AbD Serotec, Oxford, UK) and stained with fluorescence dye DAPI , . .. Statistical analysis was performed using GraphPad Prism software version 9.0 (GraphPad Software, San Diego, USA).

    Article Title: DPP4-inhibition reduces pro-inflammatory cytokine production by alpha-beta and gamma-delta T cells in vitro and in the biliary atresia mouse model.
    Article Snippet: .. Liver sections were processed as previously described with antibodies rat anti-mouse GR-1, rat anti-mouse F4/80 and anti-CD3 (Clone: 145-2C11) (AbD Serotec, Oxford, UK) and stained with fluorescence dye DAPI16,32. .. Statistical analysis was performed using GraphPad Prism software version 9.0 (GraphPad Software, San Diego, USA).

    Incubation:

    Article Title: ETS1 Orchestrates a Hybrid EMT Program Driving in vivo Metastasis and Immune Evasion
    Article Snippet: Slides were blocked with Rodent Block M (BioCareMedical, RBM961) for 30 minutes at room temperature. .. Slides were incubated with an anti-CD3 (1:100, BioRad, MCA1477) for 2 hours at room temperature and detected using the Rat-on-Mouse AP-Polymer system (BioCareMedical, RT518). .. Slides were then stained using the IntelliPATH Fast Red Chromogen Kit (BioCareMedical, IPK5017).

    Fluorescence:

    Article Title: DPP4-inhibition reduces pro-inflammatory cytokine production by alpha-beta and gamma-delta T cells in vitro and in the biliary atresia mouse model
    Article Snippet: .. Liver sections were processed as previously described with antibodies rat anti-mouse GR-1, rat anti-mouse F4/80 and anti-CD3 (Clone: 145-2C11) (AbD Serotec, Oxford, UK) and stained with fluorescence dye DAPI , . .. Statistical analysis was performed using GraphPad Prism software version 9.0 (GraphPad Software, San Diego, USA).

    Article Title: DPP4-inhibition reduces pro-inflammatory cytokine production by alpha-beta and gamma-delta T cells in vitro and in the biliary atresia mouse model.
    Article Snippet: .. Liver sections were processed as previously described with antibodies rat anti-mouse GR-1, rat anti-mouse F4/80 and anti-CD3 (Clone: 145-2C11) (AbD Serotec, Oxford, UK) and stained with fluorescence dye DAPI16,32. .. Statistical analysis was performed using GraphPad Prism software version 9.0 (GraphPad Software, San Diego, USA).



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    Nano flow cytometry measures of MV phenotype in males and females. (a) <t>CD3+</t> lymphocyte‐derived MVs. (b) CD14+ monocyte‐derived MVs. (c) CD16+ neutrophil‐derived MVs. (d) CD45+ leukocyte‐derived MVs. (e) CD31+ endothelial cell‐derived MVs. (f) CD62E+ endothelial activation‐derived MVs. (g) CD41+ platelet‐derived MVs. (h) MVs expressing Annexin A5. Individual data points are presented as MV counts/μL. Clear circles represent females and dark filled circles represent males. Statistical comparisons between sexes were performed using linear models adjusted for age and BMI. Data are presented as untransformed values for visualization following outlier removal using a 3*SD criterion. Statistical analyses were performed on log‐transformed data where appropriate.
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    Nano flow cytometry measures of MV phenotype in males and females. (a) <t>CD3+</t> lymphocyte‐derived MVs. (b) CD14+ monocyte‐derived MVs. (c) CD16+ neutrophil‐derived MVs. (d) CD45+ leukocyte‐derived MVs. (e) CD31+ endothelial cell‐derived MVs. (f) CD62E+ endothelial activation‐derived MVs. (g) CD41+ platelet‐derived MVs. (h) MVs expressing Annexin A5. Individual data points are presented as MV counts/μL. Clear circles represent females and dark filled circles represent males. Statistical comparisons between sexes were performed using linear models adjusted for age and BMI. Data are presented as untransformed values for visualization following outlier removal using a 3*SD criterion. Statistical analyses were performed on log‐transformed data where appropriate.
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    Image Search Results


    Nano flow cytometry measures of MV phenotype in males and females. (a) CD3+ lymphocyte‐derived MVs. (b) CD14+ monocyte‐derived MVs. (c) CD16+ neutrophil‐derived MVs. (d) CD45+ leukocyte‐derived MVs. (e) CD31+ endothelial cell‐derived MVs. (f) CD62E+ endothelial activation‐derived MVs. (g) CD41+ platelet‐derived MVs. (h) MVs expressing Annexin A5. Individual data points are presented as MV counts/μL. Clear circles represent females and dark filled circles represent males. Statistical comparisons between sexes were performed using linear models adjusted for age and BMI. Data are presented as untransformed values for visualization following outlier removal using a 3*SD criterion. Statistical analyses were performed on log‐transformed data where appropriate.

    Journal: Physiological Reports

    Article Title: Sex differences in circulating platelet‐derived CD41 + extracellular vesicles in healthy adults

    doi: 10.14814/phy2.70932

    Figure Lengend Snippet: Nano flow cytometry measures of MV phenotype in males and females. (a) CD3+ lymphocyte‐derived MVs. (b) CD14+ monocyte‐derived MVs. (c) CD16+ neutrophil‐derived MVs. (d) CD45+ leukocyte‐derived MVs. (e) CD31+ endothelial cell‐derived MVs. (f) CD62E+ endothelial activation‐derived MVs. (g) CD41+ platelet‐derived MVs. (h) MVs expressing Annexin A5. Individual data points are presented as MV counts/μL. Clear circles represent females and dark filled circles represent males. Statistical comparisons between sexes were performed using linear models adjusted for age and BMI. Data are presented as untransformed values for visualization following outlier removal using a 3*SD criterion. Statistical analyses were performed on log‐transformed data where appropriate.

    Article Snippet: Panel 2 received 2 μL each of CD3 PE (130‐114‐519, Miltenyi Biotec), CD14 PerCP‐Vio700 (130‐110‐523), CD16 PE‐Vio615 (130‐119‐995), CD31 FITC (130‐110‐668), CD41 APC (130‐123‐301), and 10 μL of CD62E PE (130‐104‐643).

    Techniques: Flow Cytometry, Derivative Assay, Activation Assay, Expressing, Transformation Assay